High-Throughput In Vitro ADME Analysis with Agilent RapidFire/MS Systems: Cytochrome P450 Inhibition

High-Throughput In Vitro ADME Analysis with Agilent RapidFire/MS Systems: Cytochrome P450 Inhibition

High-Throughput Lead Discovery with Agilent RapidFire/MS Systems: Analysis of Stearoyl-Coenzyme A Desaturase (SCD)

The RapidFire High-throughput Mass Spectrometry System provides drug discovery researchers with mass spectrometry-based, high-throughput screening solutions for targets that have proven challenging to screen using conventional approaches. These intractable targets have substrates and products that are either too small to label or undergo modifi cations that are diffi cult to detect. RapidFire t...

High-Throughput Lead Discovery with Agilent RapidFire/MS Systems: Analysis of Acetyl-Coenzyme A Carboxylase (ACC)

The RapidFire High-throughput Mass Spectrometry System provides drug discovery researchers with mass spectrometry-based high-throughput screening solutions for targets that have proven challenging to screen using conventional approaches. These intractable targets have substrates and products that are either too small to label or undergo modifi cations that are diffi cult to detect. RapidFire te...

High-Throughput SISCAPA-Based Peptide Quantitation Using an Agilent RapidFire High-Throughput Mass Spectrometry System

A high-throughput SISCAPA workfl ow has been developed that uses RapidFire/MS technology to enable sensitive quantitation of peptides from plasma digests with a sample cycle time of approximately 10 seconds. The optimized system includes capture of target proteotypic peptides using rigorously selected, high affi nity antipeptide monoclonal antibodies and reduction of background peptides using a...

High-throughput Analysis of DNA Modifying Enzymes by an Agilent RapidFire 360 System

Enzymes that act on DNA are promising targets for anticancer therapeutics1. For example, the epigenetic enzyme DNMT1 is inhibited by 5-azacytidine, causing DNA hypomethylation and enhancing the function of tumor suppressor genes2. In concordance, the development of accurate and user-friendly assays to study DNA modifications has become pivotal to drug discovery. Traditional in vitro assays have...